"You must use a smart card to sign in" is seen starting a published application on Windows 2016.
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Article ID: CTX269662
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Updated On:
Description
Smart card authentication is enforced on certain VDAs in the environment by
- "Interactive logon: Require smart card" located in Computer Configuration\Windows Settings\Security Settings\Local Policies\Security Options.
Or
- "Smart card is required for interactive logon" checkbox in the account tab of the user properties in Active Directory.
On a Windws 2012 R2 VDA this works as designed .

After clicking OK the follwing logon screen is displayed where a smart card tile is visible to allow smart card logon.

However on Windows 2016 after clicking on OK the session disappears and no logon screen appears.
issue only affects published applications and not published applications.
Environment
Caution! Using Registry Editor incorrectly can cause serious problems that might require you to reinstall your operating system. Citrix cannot guarantee that problems resulting from the incorrect use of Registry Editor can be solved. Use Registry Editor at your own risk. Be sure to back up the registry before you edit it.
Resolution
The issue is addressed with MS fix KB4580390.
KB4580390 is not available for Windows Server 2016 at the moment. Please upgrade to Windows Server 2019 to be able to install KB4580390.
Problem Cause
OS Change between Windows 2012 R2 and windows 2016.
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