App Layering 4.8 and before is not compatible with networking changes in Nutanix AHV 5.5.  Depending on the operation you are performing, you may see any of these errors:

Failed to connect to the server at 'host' 
A failure occurred connecting to the Nutanix server. Error = write EPROTO 140283234211648:error:14094410:SSL routines:ssl3_read_bytes:sslv3 alert handshake failure:../deps/openssl/openssl/ssl/s3_pkt.c:1472:SSL alert number 40 140283234211648:error:1409E0E5:SSL routines:ssl3_write_bytes:ssl handshake failure:../deps/openssl/openssl/ssl/s3_pkt.c:656: 
Failed to execute the script: A failure occurred connecting to the Nutanix server. Error = write EPROTO 140283234211648:error:14077410:SSL routines:SSL23_GET_SERVER_HELLO:sslv3 alert handshake failure:../deps/openssl/openssl/ssl/s23_clnt.c:769:

Upgrade to App Layering 4.9 or above.  

Starting in Nutanix Acropolis OS (AOS) 5.5, the App Layering Connector for Nutanix in ELM version 4.8 and below can no longer negotiate a secure connection to the Controller VM for management, or uploading and downloading virtual disks. As of AOS 5.5, Nutanix has disabled TLS 1.1, TLS 1.0, SSLv3 and any non-ephemeral cipher suites. The Nutanix Connector in App Layering is restricted to SSLv3 under most circumstances. Nutanix has no way to re-enable SSLv3 for compatibility with App Layering.