Follow the below steps to remove the duplicate SPN's
To identify the duplicate SPN:
1. Log on to the DDC which has the problem.
2. Click Start, point to All Programs, click Accessories, right-click Command Prompt, and then click Run as administrator.
3. If the User Account Control dialog box appears, confirm that the action it displays is what you want, and then click Continue.
4. Type setspn -X or csvde -f C:\Temp\spn.csv -d ” dc=domain,dc=in” -r “(ServicePrincipalName=*computer_name*)” -l “cn” (Above command will return all the object which contain servername on ServicePrincipalName attribute on domain.in Domain).
5. The output of this command will show the duplicate SPNs.
6. Use the following procedure to remove one of the duplicate SPNs.
To remove an SPN:
1. Click Start, point to All Programs, click Accessories, right-click Command Prompt, and then click Run as administrator.
2. If the User Account Control dialog box appears, confirm that the action it displays is what you want, and then click Continue.
3. Type setspn -D<SPN> <computer_name>, where SPN is the name of the duplicate SPN and computer_name is the name of the computer that is assigned the duplicate SPN.
Problem Cause
The issue was caused because of the Duplicate SPN for the DDC computer name for http because of which the authentication was failing.
In the Wireshark traces you will see the Kerberos error "KRB5KRB_AP_ERR_MODIFIED"