If you observe issue on the VDAs with Antivirus enabled, configure all the Antivirus exclusions as perĀ https://docs.citrix.com/en-us/tech-zone/build/tech-papers/antivirus-best-practices.html
If you are using Trend Micro, please upgraded to v20 or Later.
It is a security feature when CtxUvi.sys hooking driver detects any tampering with (meaning modification of) any of the Citrix Hook registry hives or Citrix Hook DLL files, it immediately disables all Citrix Hooking. In such a case, the symtoms could be published Desktop session stuck at grey screen on session launch.