User can access to Published app locally. .User cannot access published apps or desktop through VPN from client machine.Ping and access Domain server, local DNS, etc working fine.
Solution:
Create the Resgitry key as below as we are seeing MTU packet reset happens through UDP over the VPN network. Enabling the MTU discovery will resolve the issue.
To enable or disable EDT MTU Discovery on the VDA
To disable EDT MTU Discovery, delete this registry value and restart the VDA