XenApp and XenDesktop are placed behind the NetScaler Gateway. User attempts to access the application and get the below error message:
Unable to launch applications. Contact your helpdesk.

Based on the working and Non Working Trace.

Working Trace : SNIP sends SYN to the Store Front Server MAC Address which is the Virtual Machine.
                           SYN-ACK is from the same MAC address and here the destination is SNIP.
                           SNIP again sends ACK to the Store Front Server MAC address.

Non-Working Trace : SNIP Sends SYN to the Store Front Server MAC Address which is the Virtual Machine. 
                                  SYN-ACK is from the PHYSICAL Server MAC address where the Storefront Server is placed.
                                   As the SYN-ACK is from the other MAC address which is not expected , User will not be able to access the XA/XD which is published under Virtual Storefront Server.

This behavior is seen when NetScaler has MBF enabled where NetScaler only refers the source MAC address of the packet and it responds back to the same MAC address instead of looking into the ARP/Route table. Since response coming from the backend server has teaming address instead of server MAC address, NetScaler responding to the teaming address which was dropped in the Network

To resolve this issue disabled the teaming on the Hyper-V level.

XenApp and XenDesktop are placed behind the NetScaler Gateway. User attempts to access the application and get the below error message: Unable to launch applications. Contact your helpdesk.