After upgrading one of the servers to XenApp VDA 7.16, users with Receiver version 4.9 are unable to connect. Older receiver versions work fine, also upgrading 4.9 to 4.10 solves the issue. Receiver 4.9 connects fine to VDA 7.15. The issue only occurs when connecting through NetScaler. Internal client with Receiver 4.9 can connect fine to VDA 7.15. So it's a NetScaler issue. NetScaler is at it's latest version: NS12.0 53.13.nc